- 积分
- 15721
好友
记录
日志
相册
回帖0
主题
分享
精华
威望 旺
钢镚 分
推荐 人
|
(|[minus]|)-Epigallocatechin-3-gallate Inhibits Pancreatic Lipase and Reduces Body Weight Gain in High Fat-Fed Obese Mice
- [3 K! U% t6 i- q7 g7 Z( l
! @4 D/ ?7 P: G' [ h; w( ~6 ZKimberly A. Grove, Sudathip Sae-tan, Mary J. Kennett, Joshua D. Lambert
# l, i& B6 P$ m$ R5 ^+ W$ T' w0 P/ _ U& G
Tea (Camellia sinensis, Theaceae) has been shown to have obesity preventive effects in laboratory studies. We hypothesized that dietary epigallocatechin-3-gallate (EGCG) could reverse metabolic syndrome in high fat-fed obese C57bl/6J mice, and that these effects were related to inhibition of pancreatic lipase (PL). Following treatment with 0.32% EGCG for 6 weeks, a 44% decrease in body weight (BW) gain in high fat-fed, obese mice (P < 0.01) was observed compared to controls. EGCG treatment increased fecal lipid content by 29.4% (P < 0.05) compared to high fat-fed control, whereas in vitro, EGCG dose-dependently inhibited PL (IC50 = 7.5 μmol/l) in a noncompetitive manner with respect to substrate concentration. (−)-Epicatechin-3-gallate exhibited similar inhibitory activity, whereas the nonester-containing (−)-epigallocatechin did not. In conclusion, EGCG supplementation reduced final BW and BW gain in obese mice, and some of these effects may be due to inhibition of PL by EGCG.5 \# w1 j. ~; L' A
|
-
|